WebApr 29, 2024 · CUT&Tag for efficient epigenomic profiling of small samples and single cells - PMC Back to Top Skip to main content An official website of the United States … WebCUT-Tag/yiling-cuttag-pipeline.sh at main · ACLab-Bioinfo/CUT-Tag · GitHub ACLab-Bioinfo / CUT-Tag Public main CUT-Tag/yiling-cuttag-pipeline.sh Go to file Cannot retrieve contributors at this time 272 lines (245 sloc) 10.8 KB Raw Blame # ssh 192.168.1.23 rootpath=/public/workspace/stu18230130/MyCourse/NGS-02/lab1-CUTTag/
Improved CUT&RUN chromatin profiling tools eLife
To perform scCUT&Tag on thousands of cells, we coupled antibody-directed tagmentation (CUT&Tag)1 in bulk with an existing single-cell ATAC–seq protocol (10x Genomics, Fig. 1a). To reduce clumping of the nuclei during the incubations, we added 1% bovine serum albumin (BSA) to specific buffers … See more De novo identification of cell populations is one of the main strengths of single-cell technologies. Since scCUT&Tag allowed the deconvolution of … See more To validate the manual annotation of clusters, we used the adolescent mouse brain scRNA-seq atlas1. We picked the 100 most specifically expressed marker genes for selected populations, generated metagene modules … See more TF binding is notoriously difficult to profile using ChIP–seq in low input samples. Therefore, we asked whether scCUT&Tag was able to uncover … See more The breadth of the H3K4me3 mark has been linked previously to cell identity, gene expression and transcriptional consistency across a variety of cell types30. We noticed in the H3K4me3 pileup analysis that … See more WebMar 1, 2010 · The human CCCTC-binding factor, CTCF, organizes and regulates transcription of the genome by colocalizing distant DNA elements on the same and even different chromosomes. This protein consists of 11 zinc fingers flanked by polypeptide segments of unknown structure and function. holland america\u0027s smallest ship
Cut-C: cleavage under tethered nuclease for conformational …
WebCells were cultured in glucose-free media or in suspension for 1 day and subjected to immunoprecipitation with anti-CTCF antibodies and immunoblotting for phospho-RxxS/T. For quantification, the intensity of phospho-CTCF versus total CTCF in control samples are set at 1.0 (basal levels). WebLooking for online definition of CTCF or what CTCF stands for? CTCF is listed in the World's largest and most authoritative dictionary database of abbreviations and acronyms The … WebCTCF Antibody ChIP-seq grade Abs Negative IgG control Antibody portal Catalog Number Format Price C15410210-50 50 μg $360.00 Other format C15410210-10 10 μg Alternative name: MRD21 Polyclonal antibody raised in rabbit against human CTCF ( CCCTC-Binding Factor ), using 4 KLH coupled peptides. New! holland america\u0027s ms rotterdam