Webb8 feb. 2024 · For PTPN14 knockdown, shRNAs for human PTPN14 were designed and constructed in a GV112 lentiviral expression plasmid (Shanghai Genechem Co., Ltd., Shanghai, China). shRNA plasmids were constructed using PTPN14 target (5′-GGTCTACAGCAACAAACTTGT-3′´) and a scrambled sequence (5′ … WebbWe knocked down the intracellular MEG3 by two independent siRNAs. As shown in Figure 3A and B, loss of MEG3 significantly improved migration assessed by wound healing assay and invasion by transwell assays in SiHa cells. Besides, MEG3 overexpression in Hela cells inhibited invasion in vitro ( Figure 3C ).
Advance Publication P1 Original Article DOI: 10.5582/bst.2024
Webb27 mars 2024 · For downregulated lncRNA 00312, siRNA-mediated knockdown of lncRNA 00312 was obtained from Shanghai Genechem Co., Ltd. (China) with the following sequence: forward 5-GGCUGUUGGUCAUUCACAUTT-3and reverse 5-AUGUGAAUGACCAACAGCCTT-3.lncRNA 00312 expression was tested by qRT-PCR assay. Webb13 apr. 2024 · To determine whether the lysosomal membrane stabilization mediated by RIPK1 inhibition in ischemic astrocytes depends on lysosomal Hsp70.1B, we knocked … early bird chessington tickets
TRIM40 ameliorates diabetic retinopathy through suppressing ...
Webb20 mars 2024 · In vitro, the Numb gene was overexpressed or knocked down in the WB-F344 cell line by lentivirus transfection, Then, cells were subjected immunofluorescence … Webb6 apr. 2024 · SHANGHAI, April 6, 2024 /PRNewswire/ -- Shanghai Genechem Co., Ltd. (Genechem), a discovery company dedicated to novel drug target discovery and Webb30 jan. 2024 · In vivo, colitis was induced by oral administration of DSS in drinking water, and peritonitis was induced by an intraperitoneal injection of alum. Recombinant adeno-associated virus (AAV serotype 9) vector was used to establish ZFP91 knockdown mouse. Results We confirmed that panaxadiol inhibited IL-1β secretion by suppressing ZFP91 in … css triagle