WebAug 15, 2024 · Point-of-care (POC) molecular diagnostics play a crucial role in the prevention and treatment of infectious diseases. It is necessary to develop portable, easy-to-use, inexpensive and rapid molecular diagnostic tools. In this study, we proposed a lab-on-a-chip device that integrated DNA extraction, solid-phase PCR and genotyping detection. The … WebJan 1, 2010 · Often in diagnostic applications and in many emulsion PCR applications, such as relatively recent high-throughput sequencing systems (1–3), very low copy numbers of template nucleic acids are present prior to amplification.In systems where beads have been used as solid supports to facilitate a “read-out,” Solid Phase PCR (SP-PCR) or asymmetric …
Enhanced solid-phase recombinase polymerase amplification and …
WebSep 7, 2012 · We present a method for performing highly parallel PCR reactions in a picowell array (PWA) simultaneously immobilizing generated PCR products in a covalent and spatially-resolved manner onto a microscope slide via solid-phase PCR (SP-PCR). This so called PWA-SP-PCR was performed in picowell arrays f … WebA simple UV cross-linking method was used to immobilize the DNA probes on unmodified glass surface, which makes it convenient to integrate microarray with microfluidics. This solid-phase RT-PCR method combined RT amplification of extracted RNA in the liquid phase and species-specific nested PCR on the solid phase. simplex for sale in hayfields
Solid Phase PCR for Rapid Multiplex Detection of Salmonella
WebApr 13, 2024 · In recent years, the high prevalence of Salmonella has emerged as a serious threat to public safety, prompting attempts to utilize accurate, rapid, and direct methods to ensure food safety. In this study, a multifunctional platform featuring dual-mode detection channels (colorimetric-fluorescence) combined with polymer chain reaction (PCR) was … WebMar 14, 2011 · Solid-Phase PCR in Real Time. The polymerase chain reaction (PCR) has become the standard tool in molecular biology to discrimination between one or more … WebMar 1, 2001 · Abstract. Background: Preactivated solid surfaces provide new possibilities for multiple consecutive reactions in a microtiter plate format. In this study, a combination of PCR and subsequent hybridization in the same microtiter well was applied for the detection of HLA-B27 alleles.. Methods: A multiplex solid-phase PCR to amplify the HLA-B27 alleles … rayman glade of dreams