WebApr 13, 2024 · PE anti-mouse CD19 (clone 6D5) BioLegend: Cat#115507; RRID: AB_313642: PE/Dazzle 594 anti-mouse PDL1 (clone 10F.9G2) BioLegend: Cat#124323; RRID: AB_2565638: TruStain FcX™ (anti-mouse CD16/32) Antibody (clone 93) BioLegend: Cat#101320; RRID: AB_1574975: PE anti-human CD212 (IL12Rb1) WebCells were blocked at room temperature for 1 h in DPBS with 10% BSA and 10% Goat serum and then incubated with TruStain FcX™ PLUS anti-mouse CD16/32 (BioLegend;156604) …
Protocol - Cell Surface Pour Cytometry Staining Protocol
WebThis record provides an overview of wherewith to generally execution flow cytometric staining, including stairs on tissue/sample harvesting, red blood cell lysis, Fc receptor … Web口腔医学小站 口腔文献阅读与资源分享. 2024年4月3日 发表者 kimi1006 暂无评论. Programmable protein delivery with a bacterial contractile injection system Nature list of tv stations by state
TruStain FcX anti-mouse CD16/32 Antibody anti-CD16/32 - 93
WebJan 18, 2024 · Immune cells were isolated from within the BAL fluid, blocked with Human TruStain FcX (BioLegend, cat. no. 422302) to reduce nonspecific binding, and stained with the following cell staining panel for 20 min at 4°C: LIVE/DEAD Aqua Fixable Dye (Thermo Fisher Scientific, cat. no. L34957), PerCP-Cy5.5 anti-CD3 (clone 17A2; cat. no. 100218), … WebThis protocol provides on overview the how to perform flow cytometric smearing of whole blood samples, including steps on red blood cell lysis, surface marker color, and fixation. WebPrior to the staining procedure, the cells were washed and stained for live/dead dye using a Zombie Aqua fixable viability kit (BioLegend) and were subsequently blocked for the Fc … immortal characters in anime